Indian scientists reveal images of the SARS-CoV-2 virus that causes COVID-19

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New Delhi: Indian scientists have revealed a microscopic picture of the SARS-CoV-2 virus that causes COVID-19. The results were published in the latest edition of the Indian Journal of Medical Research (IJMR). The image is said to come from the throat swab specimen from the first laboratory-confirmed COVID-19 case in India that was reported in Kerala on January 30, 2020.

The latest studies by the Centers for Disease Control and Prevention (CDC) have shown that the coronavirus RNA was present on the Diamond Princess cruise ship in Japan after 17 days.

According to researchers, “SARS-CoV-2 RNA was identified on a variety of cabin surfaces of both symptomatic and asymptomatic infected passengers up to 17 days after cabin clearance on the Diamond Princess, but prior to disinfection procedures.”

“For the Diamond Princess, transmission was largely among passengers before quarantine, while post-quarantine crew infections peaked,” said the CDC.

After a person traveling on the cruise ship disembarked in Hong Kong and tested positive for the corona virus, the Japanese authorities decided to prohibit the 3,700 passengers on board from leaving the ship when it reached Yokohama in February. The ship was therefore quarantined until February 19, 2020.

When the Yokohama quarantine was finally removed and passengers were finally able to disembark, a total of 619 out of 3,700 passengers and crew were tested positive for COVID-19. The number of infected passengers subsequently rose to over 700. On the Grand Princess ship in California, the crew members were probably infected on Trip A and transmitted SARS-CoV-2 to passengers on Trip B.

“The results of tests on passengers and crew on board the Diamond Princess showed a high proportion (46.5 percent) of asymptomatic infections at the time of testing. The study examined the efforts of the Japanese and US governments to control the COVID-19 outbreaks Conquer at Carnival The Diamond Princess ship in Japan and the Grand Princess ship in California, “said the CDC, adding,” COVID-19 on cruise ships carries the risk of spreading disease quickly, leading to outbreaks in a vulnerable population , and aggressive efforts are required to include the spread. “

The research report said: “The description of a novel human coronavirus, originally known as the Wuhan coronavirus (CoV), is currently referred to as Severe Acute Respiratory Syndrome (SARS) -CoV-2 according to the latest International Committee for Taxonomy of Viruses The classification (ICTV) is probably the youngest human pneumonia virus with a high outbreak potential. “

“This novel virus was originally identified by next-generation sequencing (NGS) and was suspected to have a possible zoonotic origin. The detailed morphology and ultrastructure of this virus are still not fully known. The first is in the laboratory in India confirmed infection by SARS-CoV-2 was reported on January 30, 2020. The throat swab from this case was kept in commercially available transport medium (HiViral Transport Medium, HiMedia, Mumbai). “

It continues: “While a number of informal reports on electron microscopy of the SARS-CoV-2 are available from researchers at the University of Hong Kong, no detailed studies on ultrastructural cytopathology are available to date.”

The researchers summarized: “To the best of our knowledge, this is the first report from India to detect the SARS-CoV-2 virus using TEM directly in a throat swab specimen confirmed by PCR. Although TEM imaging is due to the particle load in the Sample was limited, we were still able to detect morphologically identifiable intact particles in the stored clinical samples without initial fixation. “

“The imaging of other samples such as stool and the use of immunoelectron microscopy techniques can improve the detection frequency of viruses in direct clinical material. This finding underscores the advantage of using conventional negative stained TEM imaging in clinical samples together with other diagnostic tests in parallel, especially in situations, in which etiological pathogens were identified, “concluded the researchers – Sharda Prasad, Varsha Potdar, Sarah Cherian, Priya Abraham, Atanu Basu and the ICMR-NIV NIC team.